Development and finite element modeling of modular organ-on-chip platforms

• Kira Byers, Biomedical Engineering, University of Delaware

• Jason Gleghorn, Biomedical Engineering, University of Delaware

Organ-on-a-chip models simulate the physiology of organs in vitro by allowing for the co-culture of multiple cellular populations and greater control of key microenvironmental factors to provide alternative methods for modeling diseases, immune responses, and drug delivery. We developed multi-compartment organ-on-a-chip systems that enable vascularization of kidney organoids to enhance the transport of oxygen and nutrients throughout the organoid increasing its growth and development. The kidney-on-a-chip mimics in vivo function by allowing interface between vascular cells and organoids. To allow the interface between the vascular cells and organoids within this kidney-on-a-chip model, a center organoid channel is bordered by two parallel vascular channels formed by semi-permeable polycarbonate membranes folded around a silicone layer that defines the overall shape. This three channel insert can then be sealed within an acrylic cassette to enable perfusion through or across each channel. We also explored an organ-on-a-chip system of the human endocervix, focusing on the interactions between vascular cells and the extracellular matrix (ECM). The system allows for placement of a suspended hydrogel modeling the ECM, with fluidic channels above and below, and a “cassette” that creates sealed fluidic channels. Computational modeling of fluid flow over the hydrogel within the microfluidic system was used to predict mechanical properties of the ECM. With FEBio software, we were able to simulate the displacement and strain of the hydrogel in response to fluid flow and varying pressures. This endocervix-on-a-chip model coupled with in silico modeling will enable us to better study immune response in sexually transmitted infections compared to current models.